› 5g3y

X-ray diffraction
1.18Å resolution

Crystal structure of adenylate kinase ancestor 1 with Zn and ADP bound

Released:
DOI: 10.2210/pdb5g3y/pdb
PDB entry 5g3y coloured by chain, front view.
PDB entry 5g3y coloured by chain, side view.
PDB entry 5g3y coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Evolutionary drivers of thermoadaptation in enzyme catalysis.
Nguyen V, Wilson C, Hoemberger M, Stiller JB, Agafonov RV, Kutter S, English J, Theobald DL, Kern D
Science 355 289-294 (2017)
PMID: 28008087

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADENYLATE KINSE Chain: A
Molecule details ›
Chain: A
Length: 226 amino acids
Theoretical weight: 25.02 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 46.917Å b: 63.341Å c: 70.457Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.132 0.15
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Evolution of Protein Structure and Stability in Global Warming.
Barik S. (2020)
3 more

1 mention without citation

Evolutionary drivers of thermoadaptation in enzyme catalysis.
Nguyen et al. (2017)