› 5g4w

X-ray diffraction
2.3Å resolution

S. enterica HisA mutant D7N, D10G, Dup13-15 (VVR) with substrate ProFAR

Released:
DOI: 10.2210/pdb5g4w/pdb
PDB entry 5g4w coloured by chain, front view.
PDB entry 5g4w coloured by chain, side view.
PDB entry 5g4w coloured by chain, top view.
Source organism: Salmonella enterica
Primary publication:
Structural and functional innovations in the real-time evolution of new (βα)8 barrel enzymes.
Newton MS, Guo X, Söderholm A, Näsvall J, Lundström P, Andersson DI, Selmer M, Patrick WM
Proc Natl Acad Sci U S A 114 4727-4732 (2017)
PMID: 28416687

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145288 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 27.43 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10372 (Residues: 1-12, 13-245; Coverage: 100%)
Gene names: STM2076, hisA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand GUO 1 x GUO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6122
Unit cell:
a: 87.012Å b: 87.012Å c: 120.457Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.202 0.199 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Metabolite-Enzyme Coevolution: From Single Enzymes to Metabolic Pathways and Networks.
Noda-Garcia et al. (2018)
6 more

2 mentions without citation

Structural and functional innovations in the real-time evolution of new (βα)8 barrel enzymes.
Newton et al. (2017)
1 more