› 5gat

Solution NMR

SOLUTION NMR STRUCTURE OF THE WILD TYPE DNA BINDING DOMAIN OF AREA COMPLEXED TO A 13BP DNA CONTAINING A CGATA SITE, 35 STRUCTURES

Released:
DOI: 10.2210/pdb5gat/pdb
PDB entry 5gat coloured by chain, ensemble of 35 models, front view.
PDB entry 5gat coloured by chain, ensemble of 35 models, side view.
PDB entry 5gat coloured by chain, ensemble of 35 models, top view.
Source organism: Aspergillus nidulans
Primary publication:
The solution structure of a fungal AREA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA factors.
Starich MR, Wikström M, Arst HN, Clore GM, Gronenborn AM
J Mol Biol 277 605-20 (1998)
PMID: 9533883

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118700 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nitrogen regulatory protein areA Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 7.37 KDa
Source organism: Aspergillus nidulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P17429 (Residues: 662-727; Coverage: 8%)
Gene names: AN8667, areA
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*AP*GP*CP*GP*AP*TP*AP*GP*AP*GP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.01 KDa
Source organism: Aspergillus nidulans
Expression system: Not provided
DNA (5'-D(*GP*TP*CP*TP*CP*TP*AP*TP*CP*GP*CP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 3.93 KDa
Source organism: Aspergillus nidulans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

The fungal GATA factors.
Scazzocchio C. (2000)
6 more