› 5gcn

Solution NMR

CATALYTIC DOMAIN OF TETRAHYMENA GCN5 HISTONE ACETYLTRANSFERASE IN COMPLEX WITH COENZYME A

Released:
DOI: 10.2210/pdb5gcn/pdb
PDB entry 5gcn coloured by chain, ensemble of 24 models, front view.
PDB entry 5gcn coloured by chain, ensemble of 24 models, side view.
PDB entry 5gcn coloured by chain, ensemble of 24 models, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Solution structure of the catalytic domain of GCN5 histone acetyltransferase bound to coenzyme A.
Lin Y, Fletcher CM, Zhou J, Allis CD, Wagner G
Nature 400 86-9 (1999)
PMID: 10403255

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone acetyltransferase GCN5 Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 19.73 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27198 (Residues: 45-210; Coverage: 40%)
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand COA 1 x COA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Histone acetyltransferases.
Roth et al. (2001)
15 more

6 mentions without citation

The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine.
Hanover et al. (2010)
5 more