› 5ggg

X-ray diffraction
3Å resolution

Crystal structure of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase form I

Released:
DOI: 10.2210/pdb5ggg/pdb
PDB entry 5ggg coloured by chain, front view.
PDB entry 5ggg coloured by chain, side view.
PDB entry 5ggg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Carbohydrate-binding domain of the POMGnT1 stem region modulates O-mannosylation sites of α-dystroglycan.
Kuwabara N, Manya H, Yamada T, Tateno H, Kanagawa M, Kobayashi K, Akasaka-Manya K, Hirose Y, Mizuno M, Ikeguchi M, Toda T, Hirabayashi J, Senda T, Endo T, Kato R
Proc Natl Acad Sci U S A 113 9280-5 (2016)
PMID: 27493216

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 578 amino acids
Theoretical weight: 65.55 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8WZA1 (Residues: 92-660; Coverage: 86%)
Gene names: MGAT1.2, POMGNT1, UNQ746/PRO1475
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P41212
Unit cell:
a: 99.917Å b: 99.917Å c: 236.104Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.241
Expression system: Homo sapiens

Quick links

Citations

9 review citations

Genetic glycoengineering in mammalian cells.
Narimatsu et al. (2021)
8 more

2 mentions without citation

Carbohydrate-binding domain of the POMGnT1 stem region modulates O-mannosylation sites of α-dystroglycan.
Kuwabara et al. (2016)
1 more