› 5gkj

X-ray diffraction
3.2Å resolution

Structure of EndoMS in apo form

Released:
DOI: 10.2210/pdb5gkj/pdb
PDB entry 5gkj coloured by chain, front view.
PDB entry 5gkj coloured by chain, side view.
PDB entry 5gkj coloured by chain, top view.
Source organism: Thermococcus kodakarensis KOD1
Primary publication:
Structure of the EndoMS-DNA Complex as Mismatch Restriction Endonuclease.
Nakae S, Hijikata A, Tsuji T, Yonezawa K, Kouyama KI, Mayanagi K, Ishino S, Ishino Y, Shirai T
Structure 24 1960-1971 (2016)
PMID: 27773688

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease NucS Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.6 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5JER9 (Residues: 1-252; Coverage: 100%)
Gene names: TK1898, nucS
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21212
Unit cell:
a: 128.22Å b: 70.12Å c: 70.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.275
Expression system: Escherichia coli

Quick links

Citations

9 review citations

DNA repair in the archaea-an emerging picture.
White et al. (2018)
8 more

1 mention without citation

Exploring the Binding Mechanism and Dynamics of EndoMS/NucS to Mismatched dsDNA.
Zhang et al. (2019)