› 5gmj

X-ray diffraction
2.99Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189335 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Golgi reassembly-stacking protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 25.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99JX3 (Residues: 2-208; Coverage: 46%)
Gene name: Gorasp2
Sequence domains: GRASP55/65 PDZ-like domain
Structure domains: Pdz3 Domain
Junctional adhesion molecule B Chains: C, D
Molecule details ›
Chains: C, D
Length: 19 amino acids
Theoretical weight: 2.22 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q9JI59 (Residues: 280-298; Coverage: 7%)
Gene names: Jam2, Vejam

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I4122
Unit cell:
a: 132.989Å b: 132.989Å c: 219.397Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.225 0.274
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis.
Ni et al. (2019)
4 more

1 mention without citation

Genetic, structural, and chemical insights into the dual function of GRASP55 in germ cell Golgi remodeling and JAM-C polarized localization during spermatogenesis.
Cartier-Michaud et al. (2017)