› 5gnf

X-ray diffraction
1.5Å resolution

Crystal structure of anti-CRISPR protein AcrF3

Released:
DOI: 10.2210/pdb5gnf/pdb
PDB entry 5gnf coloured by chain, front view.
PDB entry 5gnf coloured by chain, side view.
PDB entry 5gnf coloured by chain, top view.
Source organism: Pseudomonas phage JBD5
Primary publication:
A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses.
Wang J, Ma J, Cheng Z, Meng X, You L, Wang M, Zhang X, Wang Y
Cell Res 26 1165-1168 (2016)
PMID: 27585537

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phage protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.89 KDa
Source organism: Pseudomonas phage JBD5
Expression system: Escherichia coli
UniProt:
  • Canonical: L7P7R7 (Residues: 1-139; Coverage: 100%)
Gene name: JBD5_035
Sequence domains: Cas3 inhibitor AcrF3

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P65
Unit cell:
a: 84.944Å b: 84.944Å c: 63.155Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.192
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Anti-CRISPR: discovery, mechanism and function.
Pawluk et al. (2018)
11 more

4 mentions without citation

Keeping crispr in check: diverse mechanisms of phage-encoded anti-crisprs.
Trasanidou et al. (2019)
3 more