› 5gp9

X-ray diffraction
1.76Å resolution

Structural analysis of fatty acid degradation regulator FadR from Bacillus halodurans

Released:
DOI: 10.2210/pdb5gp9/pdb
PDB entry 5gp9 coloured by chain, front view.
PDB entry 5gp9 coloured by chain, side view.
PDB entry 5gp9 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
Structural basis of operator sites recognition and effector binding in the TetR family transcription regulator FadR.
Yeo HK, Park YW, Lee JY
Nucleic Acids Res 45 4244-4254 (2017)
PMID: 28160603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 22.28 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K8A4 (Residues: 2-195; Coverage: 100%)
Gene name: BH3102
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GOL 2 x GOL
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P3221
Unit cell:
a: 56.873Å b: 56.873Å c: 200.884Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
1 more

3 mentions without citation

A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldarius.
Wang et al. (2019)
2 more