› 5gpc

X-ray diffraction
2.8Å resolution

Structural analysis of fatty acid degradation regulator FadR from Bacillus halodurans

Released:
DOI: 10.2210/pdb5gpc/pdb
PDB entry 5gpc coloured by chain, front view.
PDB entry 5gpc coloured by chain, side view.
PDB entry 5gpc coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of operator sites recognition and effector binding in the TetR family transcription regulator FadR.
Yeo HK, Park YW, Lee JY
Nucleic Acids Res 45 4244-4254 (2017)
PMID: 28160603

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118709 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 194 amino acids
Theoretical weight: 22.28 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K8A4 (Residues: 2-195; Coverage: 100%)
Gene name: BH3102
Sequence domains:
Structure domains:
DNA (5'-D(P*CP*AP*TP*GP*AP*AP*TP*GP*AP*GP*TP*AP*TP*TP*CP*AP*TP*TP*CP*AP*T)-3') Chain: G
Molecule details ›
Chain: G
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*AP*TP*GP*AP*AP*TP*GP*AP*AP*TP*AP*CP*TP*CP*AP*TP*TP*CP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 46.5Å b: 76.935Å c: 87.022Å
α: 103.83° β: 105.48° γ: 89.64°
R-values:
R R work R free
0.208 0.205 0.266
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
1 more

4 mentions without citation

Physical and mechanical cues affecting biomaterial-mediated plasmid DNA delivery: insights into non-viral delivery systems.
Graceffa V. (2021)
3 more