› 5gr9

X-ray diffraction
2.77Å resolution

Crystal structure of PXY-TDIF/CLE41

Released:
DOI: 10.2210/pdb5gr9/pdb
PDB entry 5gr9 coloured by chain, front view.
PDB entry 5gr9 coloured by chain, side view.
PDB entry 5gr9 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of PXY-TDIF complex reveals a conserved recognition mechanism among CLE peptide-receptor pairs.
Zhang H, Lin X, Han Z, Qu LJ, Chai J
Cell Res 26 543-55 (2016)
PMID: 27055373

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183070 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Leucine-rich repeat receptor-like protein kinase TDR Chain: B
Molecule details ›
Chain: B
Length: 599 amino acids
Theoretical weight: 65.98 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9FII5 (Residues: 31-629; Coverage: 59%)
Gene names: At5g61480, MCI2.4, PXY, TDR
Sequence domains:
CLE41p Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.28 KDa
Source organism: Arabidopsis
Expression system: Not provided
UniProt:
  • Canonical: Q84W98 (Residues: 88-99; Coverage: 19%)
Gene names: At3g24770, CLE41, K7P8.35

Ligands and Environments

1 bound ligand:
Ligand NAG 8 x NAG
1 modified residue:
Ligand HYP 2 x HYP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 93.668Å b: 93.668Å c: 228.709Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.241
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

22 review citations

Receptor-Like Cytoplasmic Kinases: Central Players in Plant Receptor Kinase-Mediated Signaling.
Liang et al. (2018)
21 more

1 mention without citation

Differential CLE peptide perception by plant receptors implicated from structural and functional analyses of TDIF-TDR interactions.
Li et al. (2017)