› 5gt7

X-ray diffraction
2.05Å resolution

Crystal Structure of Arg-bound CASTOR1

Released:
DOI: 10.2210/pdb5gt7/pdb
PDB entry 5gt7 coloured by chain, front view.
PDB entry 5gt7 coloured by chain, side view.
PDB entry 5gt7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of arginine sensor CASTOR1 in arginine-bound and ligand free states.
Zhou Y, Wang C, Xiao Q, Guo L
Biochem Biophys Res Commun 508 387-391 (2019)
PMID: 30503338

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic arginine sensor for mTORC1 subunit 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 335 amino acids
Theoretical weight: 36.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8WTX7 (Residues: 1-323; Coverage: 98%)
Gene names: CASTOR1, GATSL3
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ARG 4 x ARG
Ligand MLI 6 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 92.833Å b: 83.146Å c: 97.289Å
α: 90° β: 116.39° γ: 90°
R-values:
R R work R free
0.186 0.185 0.211
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Amino acid-dependent control of mTORC1 signaling: a variety of regulatory modes.
Takahara et al. (2020)
1 more