› 5gw9

X-ray diffraction
1.65Å resolution

Crystal structure of C163, a backbone circularized G-CSF

Released:
DOI: 10.2210/pdb5gw9/pdb
PDB entry 5gw9 coloured by chain, front view.
PDB entry 5gw9 coloured by chain, side view.
PDB entry 5gw9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the backbone-circularized granulocyte colony-stimulating factor containing a short connector.
Miyafusa T, Shibuya R, Honda S
Biochem Biophys Res Commun 500 224-228 (2018)
PMID: 29634929

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Granulocyte colony-stimulating factor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 163 amino acids
Theoretical weight: 17.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09919 (Residues: 40-205; Coverage: 92%)
Gene names: C17orf33, CSF3, GCSF
Sequence domains: Granulocyte colony-stimulating factor
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P32
Unit cell:
a: 60.941Å b: 60.941Å c: 178.509Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.177 0.218
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation.
Hayes et al. (2023)

1 mention without citation

The combination of molecular docking and network pharmacology reveals the molecular mechanism of Danggui Niantong decoction in treating gout.
Liu et al. (2022)