› 5gzb

X-ray diffraction
2.7Å resolution

Crystal Structure of Transcription Factor TEAD4 in Complex with M-CAT DNA

Released:
DOI: 10.2210/pdb5gzb/pdb
PDB entry 5gzb coloured by chain, front view.
PDB entry 5gzb coloured by chain, side view.
PDB entry 5gzb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
DNA-binding mechanism of the Hippo pathway transcription factor TEAD4.
Shi Z, He F, Chen M, Hua L, Wang W, Jiao S, Zhou Z
Oncogene 36 4362-4369 (2017)
PMID: 28368398

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118723 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional enhancer factor TEF-3 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15561 (Residues: 36-139; Coverage: 24%)
Gene names: RTEF1, TCF13L1, TEAD4, TEF3
Sequence domains: TEA/ATTS domain
DNA (5'-D(*TP*TP*GP*CP*AP*TP*TP*CP*CP*TP*CP*TP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.87 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*AP*GP*AP*GP*GP*AP*AP*TP*GP*CP*AP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.07 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I4122
Unit cell:
a: 118.595Å b: 118.595Å c: 110.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

Structural and Functional Overview of TEAD4 in Cancer Biology.
Chen et al. (2020)
5 more

2 mentions without citation

Targeting the Hippo Pathway and Cancer through the TEAD Family of Transcription Factors.
Holden et al. (2018)
1 more