› 5h0p

X-ray diffraction
1.86Å resolution

Crystal structure of EF-hand protein mutant

Released:
DOI: 10.2210/pdb5h0p/pdb
PDB entry 5h0p coloured by chain, front view.
PDB entry 5h0p coloured by chain, side view.
PDB entry 5h0p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism underlying regulation of human EFhd2/Swiprosin-1 actin-bundling activity by Ser183 phosphorylation.
Park KR, An JY, Kang JY, Lee JG, Lee Y, Mun SA, Jun CD, Song WK, Eom SH
Biochem Biophys Res Commun 483 442-448 (2017)
PMID: 28011271

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EF-hand domain-containing protein D2 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96C19 (Residues: 70-184; Coverage: 48%)
Gene names: EFHD2, SWS1
Sequence domains: Allograft inflammatory factor 1
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P212121
Unit cell:
a: 36.675Å b: 51.105Å c: 53.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.196 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Swiprosin-1 deficiency impairs macrophage immune response of septic mice.
Zhang et al. (2018)
2 more