› 5h13

X-ray diffraction
1.9Å resolution

EED in complex with PRC2 allosteric inhibitor EED396

Released:
DOI: 10.2210/pdb5h13/pdb
PDB entry 5h13 coloured by chain, front view.
PDB entry 5h13 coloured by chain, side view.
PDB entry 5h13 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery and Molecular Basis of a Diverse Set of Polycomb Repressive Complex 2 Inhibitors Recognition by EED.
Li L, Zhang H, Zhang M, Zhao M, Feng L, Luo X, Gao Z, Huang Y, Ardayfio O, Zhang JH, Lin Y, Fan H, Mi Y, Li G, Liu L, Feng L, Luo F, Teng L, Qi W, Ottl J, Lingel A, Bussiere DE, Yu Z, Atadja P, Lu C, Li E, Gu J, Zhao K
PLoS One 12 e0169855 (2017)
PMID: 28072869

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131010 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polycomb protein EED Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 43.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75530 (Residues: 76-441; Coverage: 83%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand PR 1 x PR
Ligand LQA 1 x LQA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 150.67Å b: 45.97Å c: 51.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.229
Expression system: Escherichia coli

Quick links

Citations

13 review citations

WD40 repeat domain proteins: a novel target class?
Schapira et al. (2017)
12 more

3 mentions without citation

Polycomb repressive 2 complex-Molecular mechanisms of function.
Kouznetsova et al. (2019)
2 more