› 5h2c

X-ray diffraction
3.51Å resolution

Crystal structure of Saccharomyces cerevisiae Osh1 ANK - Nvj1

Released:
DOI: 10.2210/pdb5h2c/pdb
PDB entry 5h2c coloured by chain, front view.
PDB entry 5h2c coloured by chain, side view.
PDB entry 5h2c coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction.
Manik MK, Yang H, Tong J, Im YJ
Structure 25 617-629.e3 (2017)
PMID: 28319008

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153048 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Oxysterol-binding protein homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.99 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35845 (Residues: 7-274; Coverage: 23%)
Gene names: OSH1, SWH1, YAR042W, YAR044W
Sequence domains: Ankyrin repeat
Nucleus-vacuole junction protein 1 Chain: B
Molecule details ›
Chain: B
Length: 27 amino acids
Theoretical weight: 3.18 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38881 (Residues: 139-165; Coverage: 9%)
Gene names: NVJ1, VAB36, YHR195W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P212121
Unit cell:
a: 59.896Å b: 67.748Å c: 107.383Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.184 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The Oxysterol-Binding Protein Cycle: Burning Off PI(4)P to Transport Cholesterol.
Antonny et al. (2018)
8 more

1 mention without citation

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)