› 5h2d

X-ray diffraction
1.6Å resolution

Crystal structure of Osh1 ORD domain in complex with ergosterol

Released:
DOI: 10.2210/pdb5h2d/pdb
PDB entry 5h2d coloured by chain, front view.
PDB entry 5h2d coloured by chain, side view.
PDB entry 5h2d coloured by chain, top view.
Source organism: Kluyveromyces lactis NRRL Y-1140
Primary publication:
Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction.
Manik MK, Yang H, Tong J, Im YJ
Structure 25 617-629.e3 (2017)
PMID: 28319008

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 50.57 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6CUK7 (Residues: 808-1240; Coverage: 35%)
Gene name: KLLA0_C04147g
Sequence domains: Oxysterol-binding protein

Ligands and Environments

2 bound ligands:
Ligand ERG 1 x ERG
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 61.129Å b: 47.207Å c: 73Å
α: 90° β: 96.24° γ: 90°
R-values:
R R work R free
0.178 0.177 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The Oxysterol-Binding Protein Cycle: Burning Off PI(4)P to Transport Cholesterol.
Antonny et al. (2018)
8 more

1 mention without citation

Design, synthesis and fungicidal activity of isothiazole-thiazole derivatives.
Wu et al. (2018)