› 5h35

X-ray diffraction
2.64Å resolution

Crystal structures of the TRIC trimeric intracellular cation channel orthologue from Sulfolobus solfataricus

Released:
DOI: 10.2210/pdb5h35/pdb
PDB entry 5h35 coloured by chain, front view.
PDB entry 5h35 coloured by chain, side view.
PDB entry 5h35 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of the TRIC trimeric intracellular cation channel orthologues.
Kasuya G, Hiraizumi M, Maturana AD, Kumazaki K, Fujiwara Y, Liu K, Nakada-Nakura Y, Iwata S, Tsukada K, Komori T, Uemura S, Goto Y, Nakane T, Takemoto M, Kato HE, Yamashita K, Wada M, Ito K, Ishitani R, Hattori M, Nureki O
Cell Res 26 1288-1301 (2016)
PMID: 27909292

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212696 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy Chain Chains: A, F, H
Molecule details ›
Chains: A, F, H
Length: 236 amino acids
Theoretical weight: 25.36 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab Light Chain Chains: B, G, I
Molecule details ›
Chains: B, G, I
Length: 219 amino acids
Theoretical weight: 24.27 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Glycine transporter domain-containing protein Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 212 amino acids
Theoretical weight: 23.07 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q981D4 (Residues: 1-198; Coverage: 97%)
Gene name: SSO0012
Sequence domains: Glycine transporter

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand NA 1 x NA
Ligand PX4 3 x PX4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 102.734Å b: 171.391Å c: 103.265Å
α: 90° β: 117.94° γ: 90°
R-values:
R R work R free
0.213 0.211 0.252
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

2 review citations

TRIC-A regulates intracellular Ca2+ homeostasis in cardiomyocytes.
Zhou et al. (2021)
1 more

3 mentions without citation

Crystal structures of the TRIC trimeric intracellular cation channel orthologues.
Kasuya et al. (2016)
2 more