› 5h58

X-ray diffraction
3.99Å resolution

Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence

Released:
DOI: 10.2210/pdb5h58/pdb
PDB entry 5h58 coloured by chain, front view.
PDB entry 5h58 coloured by chain, side view.
PDB entry 5h58 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Structural and dynamics studies of the TetR family protein, CprB from Streptomyces coelicolor in complex with its biological operator sequence.
Bhukya H, Jana AK, Sengupta N, Anand R
J Struct Biol 198 134-146 (2017)
PMID: 28343010

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118727 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 23.72 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q7AKF2 (Residues: 1-215; Coverage: 100%)
Gene names: SC9B1.18, SCO6071, cprB
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2
DNA (5'-D(*AP*GP*GP*C*AP*GP*GP*CP*GP*GP*CP*AP*CP*GP*GP*TP*CP*TP*GP*TP*TP*GP*AP*GP*TP*TP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 27 nucleotides
Theoretical weight: 8.39 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Not provided
DNA (5'-D(*GP*AP*A*CP*TP*CP*AP*AP*CP*AP*GP*AP*CP*CP*GP*TP*GP*CP*CP*GP*CP*CP*TP*GP*CP*CP*T)-3') Chain: F
Molecule details ›
Chain: F
Length: 27 nucleotides
Theoretical weight: 8.21 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P32
Unit cell:
a: 149.81Å b: 149.81Å c: 70.46Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.217 0.282
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

2 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
1 more