› 5h5o

X-ray diffraction
2.12Å resolution

A cyclic-GMP-dependent signalling pathway regulates bacterial phytopathogenesis

Released:
DOI: 10.2210/pdb5h5o/pdb
PDB entry 5h5o coloured by chain, front view.
PDB entry 5h5o coloured by chain, side view.
PDB entry 5h5o coloured by chain, top view.
Source organism: Xanthomonas campestris pv. campestris str. B100
Primary publication:
A cyclic GMP-dependent signalling pathway regulates bacterial phytopathogenesis.
An SQ, Chin KH, Febrer M, McCarthy Y, Yang JG, Liu CL, Swarbreck D, Rogers J, Maxwell Dow J, Chou SH, Ryan RP
EMBO J 32 2430-8 (2013)
PMID: 23881098

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.5 KDa
Source organism: Xanthomonas campestris pv. campestris str. B100
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: B0RM05 (Residues: 23-151; Coverage: 37%)
Gene name: XCCB100_0261
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand PCG 2 x PCG
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P31
Unit cell:
a: 48.648Å b: 48.648Å c: 118.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.223 0.259
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4

Quick links

Citations

10 review citations

Cyclic di-GMP: second messenger extraordinaire.
Jenal et al. (2017)
9 more