› 5h6b

X-ray diffraction
2.3Å resolution

Crystal structure of a thermostable lipase from Marine Streptomyces

Released:
DOI: 10.2210/pdb5h6b/pdb
PDB entry 5h6b coloured by chain, front view.
PDB entry 5h6b coloured by chain, side view.
PDB entry 5h6b coloured by chain, top view.
Source organism: Streptomyces sp. W007
Primary publication:
Crystal structure of a lipase from Streptomyces sp. strain W007 - implications for thermostability and regiospecificity.
Zhao Z, Hou S, Lan D, Wang X, Liu J, Khan FI, Wang Y
FEBS J 284 3506-3519 (2017)
PMID: 28857479

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-124695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative secreted lipase Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.06 KDa
Source organism: Streptomyces sp. W007
Expression system: Komagataella pastoris
UniProt:
  • Canonical: H0B8D4 (Residues: 32-296; Coverage: 100%)
Gene name: SPW_1544
Sequence domains: Lipase (class 2)

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand ACT 2 x ACT
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P64
Unit cell:
a: 90.73Å b: 90.73Å c: 70.62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.206
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

Streptomyces spp. in the biocatalysis toolbox.
Spasic et al. (2018)
2 more

2 mentions without citation

Crystal structure of a lipase from Streptomyces sp. strain W007 - implications for thermostability and regiospecificity.
Zhao et al. (2017)
1 more