› 5h7n

X-ray diffraction
1.85Å resolution

Crystal structure of human NLRP12-PYD with a MBP tag

Released:
DOI: 10.2210/pdb5h7n/pdb
PDB entry 5h7n coloured by chain, front view.
PDB entry 5h7n coloured by chain, side view.
PDB entry 5h7n coloured by chain, top view.
Source organisms:
Primary publication:
Design of an expression system to enhance MBP-mediated crystallization.
Jin T, Chuenchor W, Jiang J, Cheng J, Li Y, Fang K, Huang M, Smith P, Xiao TS
Sci Rep 7 40991 (2017)
PMID: 28112203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142545 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; NACHT, LRR and PYD domains-containing protein 12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 478 amino acids
Theoretical weight: 52.56 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
  • Canonical: P59046 (Residues: 9-106; Coverage: 9%)
Gene names: JW3994, NALP12, NLRP12, PYPAF7, RNO, b4034, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand SO4 3 x SO4
Ligand EDO 9 x EDO
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 42.54Å b: 104.55Å c: 110.94Å
α: 90° β: 98.95° γ: 90°
R-values:
R R work R free
0.181 0.18 0.224
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Polyionic Tags as Enhancers of Protein Solubility in Recombinant Protein Expression.
Paraskevopoulou et al. (2018)
1 more

1 mention without citation

SPARC: Structural properties associated with residue constraints.
Neuwald et al. (2022)