› 5h88

X-ray diffraction
2.06Å resolution

Crystal structure of mRojoA mutant - T16V -P63F - W143A - L163V

Released:
DOI: 10.2210/pdb5h88/pdb
PDB entry 5h88 coloured by chain, front view.
PDB entry 5h88 coloured by chain, side view.
PDB entry 5h88 coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Brighter Red Fluorescent Proteins by Rational Design of Triple-Decker Motif.
Pandelieva AT, Baran MJ, Calderini GF, McCann JL, Tremblay V, Sarvan S, Davey JA, Couture JF, Chica RA
ACS Chem Biol 11 508-17 (2016)
PMID: 26697759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 242 amino acids
Theoretical weight: 27.62 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWW2 (Residues: 2-236; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CH6 4 x CH6

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 61.38Å b: 94.71Å c: 78.941Å
α: 90° β: 98.5° γ: 90°
R-values:
R R work R free
0.17 0.168 0.21
Expression system: Escherichia coli

Quick links

Citations

2 review citations

New imaging probes to track cell fate: reporter genes in stem cell research.
Jurgielewicz et al. (2017)
1 more

1 mention without citation

Nanobodies Right in the Middle: Intrabodies as Toolbox to Visualize and Modulate Antigens in the Living Cell.
Wagner et al. (2020)