› 5h9t

X-ray diffraction
2.89Å resolution

Crystal structure of native NalD at resolution of 2.9, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5h9t/pdb
PDB entry 5h9t coloured by chain, front view.
PDB entry 5h9t coloured by chain, side view.
PDB entry 5h9t coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Novobiocin binding to NalD induces the expression of the MexAB-OprM pump in Pseudomonas aeruginosa.
Chen W, Wang D, Zhou W, Sang H, Liu X, Ge Z, Zhang J, Lan L, Yang CG, Chen H
Mol Microbiol 100 749-58 (2016)
PMID: 26844397

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 212 amino acids
Theoretical weight: 24.55 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HY46 (Residues: 1-212; Coverage: 100%)
Gene names: PA3574, nalD
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 61.676Å b: 127.602Å c: 265.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

The Building Blocks of Antimicrobial Resistance in Pseudomonas aeruginosa: Implications for Current Resistance-Breaking Therapies.
Langendonk et al. (2021)
5 more

1 mention without citation

The predictive potential of different molecular markers linked to amikacin susceptibility phenotypes in Pseudomonas aeruginosa.
Nageeb et al. (2022)