› 5hay

X-ray diffraction
2.8Å resolution

Crystal structure of Chaetomium thermophilum Nup170 CTD Y905M L1007M L1183M V1292M mutant

Released:
DOI: 10.2210/pdb5hay/pdb
PDB entry 5hay coloured by chain, front view.
PDB entry 5hay coloured by chain, side view.
PDB entry 5hay coloured by chain, top view.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Architecture of the symmetric core of the nuclear pore.
Lin DH, Stuwe T, Schilbach S, Rundlet EJ, Perriches T, Mobbs G, Fan Y, Thierbach K, Huber FM, Collins LN, Davenport AM, Jeon YE, Hoelz A
Science 352 aaf1015 (2016)
PMID: 27081075

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoporin NUP170 Chains: A, B
Molecule details ›
Chains: A, B
Length: 586 amino acids
Theoretical weight: 66.15 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0S7B6 (Residues: 832-1416; Coverage: 41%)
Gene names: CTHT_0036270, NUP170
Sequence domains: Non-repetitive/WGA-negative nucleoporin C-terminal
Structure domains: Nucleoporin, helical domain, central subdomain

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 61.79Å b: 101.7Å c: 193.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.251
Expression system: Escherichia coli

Quick links

Citations

41 review citations

The Structure of the Nuclear Pore Complex (An Update).
Lin et al. (2019)
40 more

2 mentions without citation

Architecture of the symmetric core of the nuclear pore.
Lin et al. (2016)
1 more