› 5hbi

X-ray diffraction
1.6Å resolution

SCAPHARCA DIMERIC HEMOGLOBIN, MUTANT T72I, CO-LIGANDED FORM

Released:
DOI: 10.2210/pdb5hbi/pdb
Model geometry
Fit model/data
PDB entry 5hbi coloured by chain, front view.
PDB entry 5hbi coloured by chain, side view.
PDB entry 5hbi coloured by chain, top view.
Source organism: Anadara inaequivalvis
Primary publication:
Mutational destabilization of the critical interface water cluster in Scapharca dimeric hemoglobin: structural basis for altered allosteric activity.
Pardanani A, Gambacurta A, Ascoli F, Royer WE
J Mol Biol 284 729-39 (1998)
PMID: 9826511

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 15.98 KDa
Source organism: Anadara inaequivalvis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02213 (Residues: 1-146; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand CMO 2 x CMO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 93.27Å b: 43.75Å c: 83.35Å
α: 90° β: 121.93° γ: 90°
R-values:
R R work R free
0.194 0.194 0.243
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Nonvertebrate hemoglobins: functions and molecular adaptations.
Weber et al. (2001)
5 more