› 5hbu

X-ray diffraction
2.6Å resolution

Structure of the E. coli nucleoid occlusion protein SlmA bound to DNA and the C-terminal tail of the cytoskeletal cell division protein FtsZ

Released:
DOI: 10.2210/pdb5hbu/pdb
PDB entry 5hbu coloured by chain, front view.
PDB entry 5hbu coloured by chain, side view.
PDB entry 5hbu coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.
Schumacher MA, Zeng W
Proc Natl Acad Sci U S A 113 4988-93 (2016)
PMID: 27091999

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118733 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Nucleoid occlusion factor SlmA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 196 amino acids
Theoretical weight: 22.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C093 (Residues: 7-198; Coverage: 97%)
Gene names: JW5641, b3641, slmA, ttk, yicB
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2
Cell division protein FtsZ Chain: K
Molecule details ›
Chain: K
Length: 10 amino acids
Theoretical weight: 1.22 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P0A9A6 (Residues: 370-379; Coverage: 3%)
Gene names: JW0093, b0095, ftsZ, sfiB, sulB
DNA (5'-D(*GP*TP*GP*AP*GP*TP*AP*CP*TP*CP*AP*C)-3') Chains: R, T, W, Z
Molecule details ›
Chains: R, T, W, Z
Length: 12 nucleotides
Theoretical weight: 3.66 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 70.114Å b: 158.893Å c: 200.201Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

At the Heart of Bacterial Cytokinesis: The Z Ring.
Du et al. (2019)
7 more

2 mentions without citation

Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.
Schumacher et al. (2016)
1 more