› 5hca

X-ray diffraction
2.15Å resolution

Globular Domain of the Entamoeba histolytica calreticulin in complex with glucose

Released:
DOI: 10.2210/pdb5hca/pdb
PDB entry 5hca coloured by chain, front view.
PDB entry 5hca coloured by chain, side view.
PDB entry 5hca coloured by chain, top view.
Source organism: Entamoeba histolytica HM-1:IMSS
Primary publication:
Structures of parasite calreticulins provide insights into their flexibility and dual carbohydrate/peptide-binding properties.
Moreau C, Cioci G, Iannello M, Laffly E, Chouquet A, Ferreira A, Thielens NM, Gaboriaud C
IUCrJ 3 408-419 (2016)
PMID: 27840680

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calreticulin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 274 amino acids
Theoretical weight: 31.47 KDa
Source organism: Entamoeba histolytica HM-1:IMSS
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83003 (Residues: 12-202, 287-357; Coverage: 69%)
Gene names: CRT, EHI_136160
Structure domains: Jelly Rolls

Ligands and Environments

6 bound ligands:
Ligand CA 3 x CA
Ligand SO4 5 x SO4
Ligand NH4 1 x NH4
Ligand CL 2 x CL
Ligand ACT 1 x ACT
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 74.36Å b: 143.44Å c: 171.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Effects of calreticulin mutations on cell transformation and immunity.
Desikan et al. (2023)
3 more

4 mentions without citation

Calnexin cycle - structural features of the ER chaperone system.
Kozlov et al. (2020)
3 more