5hcm

X-ray diffraction
3.15Å resolution

The GLIC pentameric Ligand-Gated Ion Channel 2-21' cross-linked mutant complexed to bromoform

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 317 amino acids
Theoretical weight: 36.24 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 44-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 177.268Å b: 130.934Å c: 155.76Å
α: 90° β: 100.31° γ: 90°
R-values:
R R work R free
0.231 0.23 0.24
Expression system: Escherichia coli