› 5hdp

X-ray diffraction
2.9Å resolution

Hydrolase StnA mutant - S185A

Released:
DOI: 10.2210/pdb5hdp/pdb
PDB entry 5hdp coloured by chain, front view.
PDB entry 5hdp coloured by chain, side view.
PDB entry 5hdp coloured by chain, top view.
Source organism: Streptomyces albus
Primary publication:
Crystal Structure of StnA for the Biosynthesis of Antitumor Drug Streptonigrin Reveals a Unique Substrate Binding Mode.
Qian T, Wo J, Zhang Y, Song Q, Feng G, Luo R, Lin S, Wu G, Chen HF
Sci Rep 7 40254 (2017)
PMID: 28074848

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125834 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 383 amino acids
Theoretical weight: 40.98 KDa
Source organism: Streptomyces albus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: L7PIJ2 (Residues: 2-375; Coverage: 100%)
Gene name: stnA
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

1 bound ligand:
Ligand STM 7 x STM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 83.256Å b: 92.847Å c: 104.037Å
α: 115.06° β: 106.09° γ: 97.69°
R-values:
R R work R free
0.246 0.244 0.298
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 citation in other articles

Robustness and Efficiency of Poisson-Boltzmann Modeling on Graphics Processing Units.
Qi et al. (2019)
2 more

1 mention without citation

Crystal Structure of StnA for the Biosynthesis of Antitumor Drug Streptonigrin Reveals a Unique Substrate Binding Mode.
Qian et al. (2017)