› 5heo

X-ray diffraction
3.3Å resolution

Pentameric ligand-gated ion channel ELIC mutant P254G

Released:
DOI: 10.2210/pdb5heo/pdb
PDB entry 5heo coloured by chain, front view.
PDB entry 5heo coloured by chain, side view.
PDB entry 5heo coloured by chain, top view.
Source organism: Dickeya dadantii 3937
Primary publication:
Signal Transduction at the Domain Interface of Prokaryotic Pentameric Ligand-Gated Ion Channels.
Bertozzi C, Zimmermann I, Engeler S, Hilf RJ, Dutzler R
PLoS Biol 14 e1002393 (2016)
PMID: 26943937

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122692 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 322 amino acids
Theoretical weight: 36.84 KDa
Source organism: Dickeya dadantii 3937
Expression system: Escherichia coli
UniProt:
  • Canonical: E0SJQ4 (Residues: 22-343; Coverage: 100%)
Gene name: Dda3937_00520
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 104.95Å b: 266.57Å c: 110.69Å
α: 90° β: 109.33° γ: 90°
R-values:
R R work R free
0.222 0.22 0.252
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
20 other articles

1 mention without citation

X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels.
Chen et al. (2018)