› 5hgz

X-ray diffraction
1.38Å resolution

Crystal structure of human Naa60 in complex with acetyl-CoA

Released:
DOI: 10.2210/pdb5hgz/pdb
PDB entry 5hgz coloured by chain, front view.
PDB entry 5hgz coloured by chain, side view.
PDB entry 5hgz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase.
Chen JY, Liu L, Cao CL, Li MJ, Tan K, Yang X, Yun CH
Sci Rep 6 31425 (2016)
PMID: 27550639

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-[transmembrane protein] + CoA
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190667 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-alpha-acetyltransferase 60 Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H7X0 (Residues: 1-242; Coverage: 100%)
Gene names: HAT4, NAA60, NAT15, UNQ2771/PRO7155
Sequence domains: Acetyltransferase (GNAT) family

Ligands and Environments


Cofactor: Ligand ACO 1 x ACO
1 bound ligand:
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 53.287Å b: 57.365Å c: 68.818Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.192
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Protein N-Terminal Acetylation: Structural Basis, Mechanism, Versatility, and Regulation.
Deng et al. (2021)
5 more

3 mentions without citation

Structure and function of human Naa60 (NatF), a Golgi-localized bi-functional acetyltransferase.
Chen et al. (2016)
2 more