› 5hhl

X-ray diffraction
2.1Å resolution

Reverse transcriptase domain of group II intron maturase from Eubacterium rectale in P21 space group

Released:
DOI: 10.2210/pdb5hhl/pdb
PDB entry 5hhl coloured by chain, front view.
PDB entry 5hhl coloured by chain, side view.
PDB entry 5hhl coloured by chain, top view.
Source organism: [Eubacterium] rectale M104/1
Primary publication:
Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.
Zhao C, Pyle AM
Nat Struct Mol Biol 23 558-65 (2016)
PMID: 27136328

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retron-type reverse transcriptase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 292 amino acids
Theoretical weight: 33.22 KDa
Source organism: [Eubacterium] rectale M104/1
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand K 8 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 74.83Å b: 119.86Å c: 161.47Å
α: 90° β: 92.08° γ: 90°
R-values:
R R work R free
0.203 0.201 0.238
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Using bioinformatic and phylogenetic approaches to classify transposable elements and understand their complex evolutionary histories.
Arkhipova IR. (2017)
15 more

5 mentions without citation

Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.
Zhao et al. (2016)
4 more