› 5hjz

X-ray diffraction
1.98Å resolution

Structure of M. tuberculosis MazF-mt1 (Rv2801c) in complex with RNA

Released:
DOI: 10.2210/pdb5hjz/pdb
PDB entry 5hjz coloured by chain, front view.
PDB entry 5hjz coloured by chain, side view.
PDB entry 5hjz coloured by chain, top view.
Source organisms:
Entry authors: Yen TJ, Brennan RG

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159810 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Endoribonuclease MazF9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 12.98 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P71650 (Residues: 1-118; Coverage: 100%)
Gene names: Rv2801c, mazF-mt1, mazF9
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
RNA (5'-R(*CP*AP*UP*C)-D(P*U)-R(P*AP*CP*CP*UP*GP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 38.799Å b: 68.542Å c: 80.162Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.23
Expression system: Escherichia coli

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