› 5hk7

X-ray diffraction
2.95Å resolution

Bacterial sodium channel pore, 2.95 Angstrom resolution

Released:
DOI: 10.2210/pdb5hk7/pdb
Model geometry
Fit model/data
PDB entry 5hk7 coloured by chain, front view.
PDB entry 5hk7 coloured by chain, side view.
PDB entry 5hk7 coloured by chain, top view.
Source organism: Alkalilimnicola ehrlichii
Primary publication:
Unfolding of a Temperature-Sensitive Domain Controls Voltage-Gated Channel Activation.
Arrigoni C, Rohaim A, Shaya D, Findeisen F, Stein RA, Nurva SR, Mishra S, Mchaourab HS, Minor DL
Cell 164 922-36 (2016)
PMID: 26919429

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ion transport domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 152 amino acids
Theoretical weight: 17.46 KDa
Source organism: Alkalilimnicola ehrlichii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0ABW0 (Residues: 143-288; Coverage: 51%)
Gene name: Mlg_0322
Sequence domains:
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand VVA 10 x VVA
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 153.51Å b: 160.52Å c: 166.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.236
Expression system: Escherichia coli

Quick links

Citations

8 review citations

New Structures and Gating of Voltage-Dependent Potassium (Kv) Channels and Their Relatives: A Multi-Domain and Dynamic Question.
Barros et al. (2019)
7 more

7 mentions without citation

Global versus local mechanisms of temperature sensing in ion channels.
Arrigoni et al. (2018)
6 more