› 5hlh

X-ray diffraction
3Å resolution

Crystal structure of the overoxidized AbfR bound to DNA

Released:
DOI: 10.2210/pdb5hlh/pdb
PDB entry 5hlh coloured by chain, front view.
PDB entry 5hlh coloured by chain, side view.
PDB entry 5hlh coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into the Redox-Sensing Mechanism of MarR-Type Regulator AbfR.
Liu G, Liu X, Xu H, Liu X, Zhou H, Huang Z, Gan J, Chen H, Lan L, Yang CG
J Am Chem Soc 139 1598-1608 (2017)
PMID: 28086264

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118744 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH marR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 147 amino acids
Theoretical weight: 17.46 KDa
Source organism: Staphylococcus epidermidis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HKZ1 (Residues: 1-146; Coverage: 100%)
Gene name: SERP2196
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*TP*AP*AP*CP*TP*CP*AP*AP*TP*CP*GP*CP*GP*CP*GP*CP*GP*AP*TP*TP*GP*AP*GP*T)-3') Chains: I, J, K, L, M, N, O, P
Molecule details ›
Chains: I, J, K, L, M, N, O, P
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand OCS 8 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 150.305Å b: 290.914Å c: 52.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.272
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Functional Mechanism of the Efflux Pumps Transcription Regulators From Pseudomonas aeruginosa Based on 3D Structures.
Housseini B Issa et al. (2018)
1 more