› 5hlx

X-ray diffraction
1.5Å resolution

X-ray crystal structure of met F43H/H64A sperm whale myoglobin in complex with nitrite

Released:
DOI: 10.2210/pdb5hlx/pdb
PDB entry 5hlx coloured by chain, front view.
PDB entry 5hlx coloured by chain, side view.
PDB entry 5hlx coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Regulating the nitrite reductase activity of myoglobin by redesigning the heme active center.
Wu LB, Yuan H, Gao SQ, You Y, Nie CM, Wen GB, Lin YW, Tan X
Nitric Oxide 57 21-29 (2016)
PMID: 27108710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.16 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand NO2 2 x NO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 39.701Å b: 49.545Å c: 77.588Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.173
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Design of artificial metalloproteins/metalloenzymes by tuning noncovalent interactions.
Hirota et al. (2018)
13 other articles

1 mention without citation

Water stabilizes an alternate turn conformation in horse heart myoglobin.
Bronstein et al. (2023)