› 5hr9

X-ray diffraction
2.2Å resolution

The crystal structure of Se-AsfvPolX(L52/163M mutant) in complex with 1nt-gap DNA1

Released:
DOI: 10.2210/pdb5hr9/pdb
PDB entry 5hr9 coloured by chain, front view.
PDB entry 5hr9 coloured by chain, side view.
PDB entry 5hr9 coloured by chain, top view.
Source organisms:
Entry authors: Chen YQ, Zhang J, Gan JH

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118754 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Repair DNA polymerase X Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.83 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Sequence domains:
Structure domains:
DNA (5'-D(*GP*GP*AP*CP*AP*AP*CP*GP*GP*GP*AP*CP*AP*AP*C)-3') Chains: C, F
Molecule details ›
Chains: C, F
Length: 15 nucleotides
Theoretical weight: 4.64 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*GP*TP*TP*GP*TP*CP*C)-3') Chains: D, E, G, H
Molecule details ›
Chains: D, E, G, H
Length: 7 nucleotides
Theoretical weight: 2.1 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 74.323Å b: 82.24Å c: 101.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links