› 5hrd

X-ray diffraction
1.8Å resolution

The crystal structure of AsfvPolX:DNA2 binary complex

Released:
DOI: 10.2210/pdb5hrd/pdb
PDB entry 5hrd coloured by chain, front view.
PDB entry 5hrd coloured by chain, side view.
PDB entry 5hrd coloured by chain, top view.
Source organisms:
Entry authors: Chen YQ, Zhang J, Gan JH

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118756 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Repair DNA polymerase X Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 178 amino acids
Theoretical weight: 20.61 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*AP*TP*CP*GP*G)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 60.196Å b: 87.485Å c: 87.914Å
α: 90° β: 91.45° γ: 90°
R-values:
R R work R free
0.199 0.196 0.245
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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