› 5hrg

X-ray diffraction
2Å resolution

The crystal structure of AsfvPolX(D51N mutant):DNA4 binary complex

Released:
DOI: 10.2210/pdb5hrg/pdb
PDB entry 5hrg coloured by chain, front view.
PDB entry 5hrg coloured by chain, side view.
PDB entry 5hrg coloured by chain, top view.
Source organisms:
Entry authors: Chen YQ, Zhang J, Gan JH

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118759 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Repair DNA polymerase X Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.61 KDa
Source organism: African swine fever virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42494 (Residues: 1-174; Coverage: 100%)
Gene names: Ba71V-97, O174L
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*GP*AP*TP*CP*GP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.43 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 44.06Å b: 51.17Å c: 55.54Å
α: 68.35° β: 86.25° γ: 88.2°
R-values:
R R work R free
0.193 0.191 0.239
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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