› 5hsa

X-ray diffraction
2.35Å resolution

Alcohol Oxidase AOX1 from Pichia Pastoris

Released:
DOI: 10.2210/pdb5hsa/pdb
PDB entry 5hsa coloured by chain, front view.
PDB entry 5hsa coloured by chain, side view.
PDB entry 5hsa coloured by chain, top view.
Source organism: Komagataella phaffii CBS 7435
Primary publication:
Crystal Structure of Alcohol Oxidase from Pichia pastoris.
Koch C, Neumann P, Valerius O, Feussner I, Ficner R
PLoS One 11 e0149846 (2016)
PMID: 26905908

Function and Biology Details

Reaction catalysed: 
A primary alcohol + O(2) = an aldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol oxidase 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 663 amino acids
Theoretical weight: 73.99 KDa
Source organism: Komagataella phaffii CBS 7435
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: F2QY27 (Residues: 1-663; Coverage: 100%)
Gene names: AOX1, PP7435_Chr4-0130
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAS 8 x FAS
6 bound ligands:
Ligand P6G 9 x P6G
Ligand PGE 9 x PGE
Ligand PO4 2 x PO4
Ligand CA 8 x CA
Ligand GOL 2 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 117.1Å b: 165.19Å c: 164.31Å
α: 90° β: 95.67° γ: 90°
R-values:
R R work R free
0.179 0.177 0.205
Expression system: Komagataella phaffii CBS 7435

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Citations

1 review citation

Advances in high-resolution cryo-EM of oligomeric enzymes.
Vonck et al. (2017)
9 other articles

9 mentions without citation

Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.
Bissaro et al. (2018)
8 more