› 5hum

X-ray diffraction
1.6Å resolution

The crystal structure of neuraminidase from A/Sichuan/26221/2014 influenza virus

Released:
DOI: 10.2210/pdb5hum/pdb
PDB entry 5hum coloured by chain, front view.
PDB entry 5hum coloured by chain, side view.
PDB entry 5hum coloured by chain, top view.
Source organism: Influenza A virus (A/chicken/Sichuan/NCJPL1/2014(H5N6))
Primary publication:
Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.
Yang H, Carney PJ, Mishin VP, Guo Z, Chang JC, Wentworth DE, Gubareva LV, Stevens J
J Virol 90 5770-5784 (2016)
PMID: 27053557

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 391 amino acids
Theoretical weight: 43.21 KDa
Source organism: Influenza A virus (A/chicken/Sichuan/NCJPL1/2014(H5N6))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A0B4N5Z5 (Residues: 82-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand NAG 8 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 117.335Å b: 76.277Å c: 117.211Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.199 0.198 0.22
Expression system: Trichoplusia ni

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Citations

4 review citations

Pandemic potential of highly pathogenic avian influenza clade 2.3.4.4 A(H5) viruses.
Yamaji et al. (2020)
3 more

1 mention without citation

Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.
Yang et al. (2016)