› 5hun

X-ray diffraction
2.3Å resolution

The crystal structure of neuraminidase from A/gyrfalcon/Washington/41088-6/2014 influenza virus

Released:
DOI: 10.2210/pdb5hun/pdb
PDB entry 5hun coloured by chain, front view.
PDB entry 5hun coloured by chain, side view.
PDB entry 5hun coloured by chain, top view.
Source organism: Influenza A virus (A/gyrfalcon/Washington/41088-6/2014(H5N8))
Primary publication:
Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.
Yang H, Carney PJ, Mishin VP, Guo Z, Chang JC, Wentworth DE, Gubareva LV, Stevens J
J Virol 90 5770-5784 (2016)
PMID: 27053557

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 43.96 KDa
Source organism: Influenza A virus (A/gyrfalcon/Washington/41088-6/2014(H5N8))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A0C4WVM3 (Residues: 80-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I4
Unit cell:
a: 90.652Å b: 90.652Å c: 110.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.237
Expression system: Trichoplusia ni

Quick links

Citations

4 review citations

Pandemic potential of highly pathogenic avian influenza clade 2.3.4.4 A(H5) viruses.
Yamaji et al. (2020)
3 more

3 mentions without citation

Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.
Yang et al. (2016)
2 more