› 5hvm

X-ray diffraction
2.82Å resolution

Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A

Released:
DOI: 10.2210/pdb5hvm/pdb
PDB entry 5hvm coloured by chain, front view.
PDB entry 5hvm coloured by chain, side view.
PDB entry 5hvm coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design.
Miao Y, Tenor JL, Toffaletti DL, Maskarinec SA, Liu J, Lee RE, Perfect JR, Brennan RG
mBio 8 (2017)
PMID: 28743811

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-175827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 515 amino acids
Theoretical weight: 57.99 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WLM9 (Residues: 1-515; Coverage: 100%)
Gene names: AFUA_6G12950, tpsA
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand UDP 2 x UDP
Ligand VDM 2 x VDM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Unit cell:
a: 83.915Å b: 101.153Å c: 146.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.176 0.23
Expression system: Escherichia coli

Quick links

Citations

2 review citations

An insight into new strategies to combat antifungal drug resistance.
Zheng et al. (2018)
1 more

2 mentions without citation

Structural and In Vivo Studies on Trehalose-6-Phosphate Synthase from Pathogenic Fungi Provide Insights into Its Catalytic Mechanism, Biological Necessity, and Potential for Novel Antifungal Drug Design.
Miao et al. (2017)
1 more