› 5hx4

X-ray diffraction
1.92Å resolution

Zinc-Free APOBEC3F Catalytic Domain Crystal Structure

Released:
DOI: 10.2210/pdb5hx4/pdb
PDB entry 5hx4 coloured by chain, front view.
PDB entry 5hx4 coloured by chain, side view.
PDB entry 5hx4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
1.92 Angstrom Zinc-Free APOBEC3F Catalytic Domain Crystal Structure.
Shaban NM, Shi K, Li M, Aihara H, Harris RS
J Mol Biol 428 2307-2316 (2016)
PMID: 27139641

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3F Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 23.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IUX4 (Residues: 185-373; Coverage: 51%)
Gene name: APOBEC3F
Sequence domains: APOBEC-like N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 36.1Å b: 51.27Å c: 99.77Å
α: 90° β: 90.85° γ: 90°
R-values:
R R work R free
0.186 0.185 0.214
Expression system: Escherichia coli

Quick links

Citations

9 review citations

APOBEC Enzymes as Targets for Virus and Cancer Therapy.
Olson et al. (2018)
8 more

3 mentions without citation

A Novel Regulator of Activation-Induced Cytidine Deaminase/APOBECs in Immunity and Cancer: Schrödinger's CATalytic Pocket.
King et al. (2017)
2 more