› 5hxa

X-ray diffraction
2Å resolution

Crystal structure of an UDP-forming alpha, alpha-terhalose-phosphate synthase from Burkholderia xenovorans

Released:
DOI: 10.2210/pdb5hxa/pdb
PDB entry 5hxa coloured by chain, front view.
PDB entry 5hxa coloured by chain, side view.
PDB entry 5hxa coloured by chain, top view.
Source organism: Paraburkholderia xenovorans LB400
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trehalose-6-phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 483 amino acids
Theoretical weight: 54.39 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q142X8 (Residues: 1-475; Coverage: 100%)
Gene name: Bxe_A3374
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
Ligand EDO 10 x EDO
Ligand GOL 1 x GOL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6422
Unit cell:
a: 171.18Å b: 171.18Å c: 95.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.157 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links