› 5hz2

X-ray diffraction
1.8Å resolution

Crystal structure of PhaC1 from Ralstonia eutropha

Released:
DOI: 10.2210/pdb5hz2/pdb
PDB entry 5hz2 coloured by chain, front view.
PDB entry 5hz2 coloured by chain, side view.
PDB entry 5hz2 coloured by chain, top view.
Source organism: Cupriavidus necator H16
Primary publication:
Crystal structure of Ralstonia eutropha polyhydroxyalkanoate synthase C-terminal domain and reaction mechanisms.
Kim J, Kim YJ, Choi SY, Lee SY, Kim KJ
Biotechnol J 12 (2017)
PMID: 27808482

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(3-hydroxyalkanoate) polymerase subunit PhaC Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.65 KDa
Source organism: Cupriavidus necator H16
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23608 (Residues: 202-589; Coverage: 66%)
Gene names: H16_A1437, phaC, phbC
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: I222
Unit cell:
a: 73.246Å b: 87.856Å c: 136.479Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Can Polyhydroxyalkanoates Be Produced Efficiently From Waste Plant and Animal Oils?
Surendran et al. (2020)
8 more

3 mentions without citation

Enzyme catalyzes ester bond synthesis and hydrolysis: The key step for sustainable usage of plastics.
Lai et al. (2022)
2 more