› 5hz7

X-ray diffraction
1.43Å resolution

High-resolution crystal structure of the minor DNA-binding pilin ComP from Neisseria meningitidis in fusion with MBP

Released:
DOI: 10.2210/pdb5hz7/pdb
PDB entry 5hz7 coloured by chain, front view.
PDB entry 5hz7 coloured by chain, side view.
PDB entry 5hz7 coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
A Comparative Structure/Function Analysis of Two Type IV Pilin DNA Receptors Defines a Novel Mode of DNA Binding.
Berry JL, Xu Y, Ward PN, Lea SM, Matthews SJ, Pelicic V
Structure 24 926-34 (2016)
PMID: 27161979

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 53.74 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand EDO 2 x EDO
Ligand BGC 1 x BGC
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 63.57Å b: 68.44Å c: 109.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.191
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Motility and adhesion through type IV pili in Gram-positive bacteria.
Piepenbrink et al. (2016)
1 more

2 mentions without citation

DNA Uptake by Type IV Filaments.
Piepenbrink KH. (2019)
1 more