› 5hza

X-ray diffraction
1.35Å resolution

Crystal structure of GII.10 P domain in complex with 3-fucosyllactose (3 FL)

Released:
DOI: 10.2210/pdb5hza/pdb
PDB entry 5hza coloured by chain, front view.
PDB entry 5hza coloured by chain, side view.
PDB entry 5hza coloured by chain, top view.
Source organism: Norovirus GII.10
Primary publication:
Structural Basis for Norovirus Inhibition by Human Milk Oligosaccharides.
Weichert S, Koromyslova A, Singh BK, Hansman S, Jennewein S, Schroten H, Hansman GS
J Virol 90 4843-4848 (2016)
PMID: 26889023

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Capsid protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.51 KDa
Source organism: Norovirus GII.10
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F4T5 (Residues: 224-538; Coverage: 58%)
Sequence domains: Calicivirus coat protein C-terminal
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, FUC, GAL
Carbohydrate polymer
1 bound ligand:
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21
Unit cell:
a: 65.71Å b: 78.17Å c: 71.84Å
α: 90° β: 102.3° γ: 90°
R-values:
R R work R free
0.162 0.161 0.179
Expression system: Escherichia coli BL21

Quick links

Citations

37 review citations

Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health.
Lyons et al. (2020)
36 more

2 mentions without citation

Immunomodulatory Properties of Human Breast Milk: MicroRNA Contents and Potential Epigenetic Effects.
Hatmal et al. (2022)
1 more